Cell Lines

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Please refer to ATCC for more detailed information.

Contents

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media & solutions

  • Eagle's MEM (minimum essential media)
  • Eagle's MEM/Ham's F12 (MEM/F12)
  • Dubecco's MEM (DMEM)
  • Iscove's modified Dulbecco's medium (IMDM)
  • McCoys 5A
  • MEM with Earle's salts
  • RPMI
  • 100X HT solution (0.1 mM hypoxanthine and 0.016 mM thymidine at 1X)


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Brain

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CHP-212

  • CRL-2273
  • neuroblast
  • maintained in MEM/F12 + 10% FBS
  • can be passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:10 - 1:50, every 2-3 weeks, replace media every few days)
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IMR-32

  • CCL-127
  • neuroblast
  • maintained in Eagle's MEM + 10% FBS
  • can be passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:6)


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Blood

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DT40

  • CRL-2111
  • chicken lymphoblast
  • maintained in RPMI + 10%FBS + 1% chicken serum + 50uM beta-mercaptoethanol
  • ATCC: 75% DMEM + 50uM beta-mercaptoethanol; 10% tryptose phosphate broth; 10% fetal bovine serum; 5% chicken serum
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S9.6

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THP1

  • RPMI-1640 Medium + 0.05mM 2-mercaptoethanol (2uL of 14.3M for 555mL) + 10% fetal bovine serum (55mL for 555mL)


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Breast

Breast Cancer Cell Line Database

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EMT6

  • CRL-2755
  • a transplantable mouse mammary carcinoma that arose in a BALB/cCRGL mouse after implantation of a hyperplastic mammary alveolar nodule
  • maintained in MEM/F12 + 10% FBS
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:10) every 3 to 4 days
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MCF7

  • HTB-22
  • mammary adenocarcinoma
  • MEM + Earle's Salts + 10% FBS
  • Trypsin. Quench with media. Transfer the cell suspension to the centrifuge tube with the medium and cells, and centrifuge for 5 minutes. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
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MCF10A

  • CRL-10317
  • non-tumorigenic epithelial cell line
  • Differentiation protocol
  • DMEM/F12 + 2.5% Horse serum + 0.5ug/mL hydrocortisone + 10ug/mL Insulin + 20ng/mL EGF + 100ng/mL Cholera toxin
  • Trypsin at 37C. Quench with media. Transfer the cell suspension to the centrifuge tube with the medium and cells, and centrifuge for 5 minutes. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
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MDA-MB-231

  • HTB-26
  • mammary adenocarcinoma
  • DMEM + 10% FBS
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:2 to 1:4)


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Cervix

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HeLa

  • cervical carcinoma
  • various lines (CCL-2, SEN2, S3)
    • CCL-2 is directly from ATCC, adherent
    • SEN2 is kindly provided by DiMaio lab, adherent
    • S3 from Maniatis Lab, suspension
  • CCL-2 and SEN2 are maintained in DMEM + 10% FBS and passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (upto 20 fold dilution is OK)
  • S3 cells are maintained in RPMI + 10% FBS and passaged by dilution (1:10)


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Colon

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CT26.WT

  • CRL-2638
  • an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated mouse colon carcinoma cell line
  • RPMI-1640 + 10% fetal bovine serum (55mL for 555mL)
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:10) every 3 to 4 days
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HCT116

  • CCL-247
  • colorectal carcinoma
  • maintained in McCoys 5A + 10% FBS
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:8) every 3 to 4 days
  • freeze medium: complete growth medium supplemented with 5% (v/v) DMSO
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HT29

  • colorectal carcinoma
  • three lines available: HT29, HT29-bGAL, HT29-APC
  • HT29-bGAL and HT29-APC are provided by Bert Vogelstein are described in Morin et al
    • HT29-bGAL and -APC lines contain zinc inducible (metallothionein promoter) beta-galactosidase or adenomatopolyposis coli transgenes, respectively
    • maintained in McCoys 5A media + 10% FBS + 0.6mg/mL Hygromycin (Invitrogen)
  • HT29 is the parental line
    • maintained in McCoys 5A media + 10% FBS
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:8) every 3 to 4 days
  • avoid prolonged passage


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Kidney

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HEK293

  • CRL-1573
  • embryonic kidney epithelial cells
  • DMEM + 10% FBS
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (upto 20 fold dilution is OK)
  • HEK293-EGFP
    • independently established in our lab, September 2007
    • HEK293 stably transfected with pEGFP C3
    • DMEM + 10% FBS + 1mg/mL G418 sulfate (HyClone)


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Lung

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IMR90

  • CCL-186
  • primary fetal lung fibroblast, adherent
  • maintained in MEM with Earles salts + 10% FBS
  • can be passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media (1:10)


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Prostate

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LnCaP

  • CRL-1740
  • prostate carcinoma
  • RPMI + 10% FBS
  • passaged by mild treatment with 0.25X trypsin/EDTA and diluting into fresh media
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