ChIP-qPCR v1.0

From Genome@Yale

Jump to: navigation, search
  1. Dilute primers to 5uM and make primer pair mixes
  2. Add 3uL primer pair mix (1.5uL each 5uM Left and Right, 0.3uM final)
  3. Add 0.5ng ChIP DNA (or 0.5ng Input DNA) to wells
  4. Bring the total volume to 12.5uL with dH2O
  5. Add 12.5uL SYBR PCR Supmermix (BioRad)
  6. Analyze on realtime PCR 
50-500pg DNA
0.1 - 1.0 uM primers
1X SYBR mastermix
dH2O to 10-25uL final
Retrieved from "http://genome.med.yale.edu/index.php/ChIP-qPCR_v1.0"
Views
internal