Protocols
From Genome@Yale
These pages are constantly updated.
Check back often for the most updated versions of these protocols
Contents |
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Online Resources
- BioTechniques Protocol Guide
- Cold Spring Harbor Protocols
- Nature Protocols
- Protocol Online
- Springer Protocols
- Wiley’s Current Protocols
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Chromatin Immunoprecipitation
- ChIP Overview
- Growing Cells
- Chromatin Isolation
- re-ChIP protocol version 1.0
- Analysis of ChIP DNA by quantitative PCR
- Complete ChIP-chip protocol from our lab, version 1.0
- ChIP-chip protocol for 10,000 cells from Farnham lab
- Agilent Array Protocols
- Solexa Sequencing Protocols
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Cell
- Cell Lines
- Cryopreservation
- Subculturing
- Transfection
- Crystal Violet Stain
- Propidium Iodide Stain
- GFP Detection
- Apoptosis Detection
- retrovirus generation
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DNA
- Reporters
- Subcloning
- DNA Sequencing
- PicoGreen dsDNA Quantitation
- PEG Precipitation
- PAGE
- Genomic DNA isolation
- DNase I hypersensitivity assay
- MIP analysis
- BAC modification
- tailored shRNA library construction
- FISH
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RNA
- RNA purification
- direct RNA labeling for hybridization
- cDNA synthesis
- realtime-PCR
- nuclear run-ons
- in vitro transcription
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Protein
- Total Protein Extraction
- Recombinant Protein
- Baculovirus Expression
- mAb purification
- SDS-PAGE
- Western Blot
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Analysis
- Sequence Analysis
- Array Analysis and Visualization
- Statistical Analysis
- Large Scale Experiment Design
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Custom Array Production
- spotted DNA arrays
- home-made hot-start Taq
- designing commercial custom arrays
